Application of chicken microarrays for gene expression analysis in other avian species

BACKGROUND: With the threat of emerging infectious diseases such as avian influenza, whose natural hosts are thought to be a variety of wild water birds including duck, we are armed with very few genomic resources to investigate large scale immunological gene expression studies in avian species. Multiple options exist for conducting large gene expression studies in chickens and in this study we explore the feasibility of using one of these tools to investigate gene expression in other avian species. RESULTS: In this study we utilised a whole genome long oligonucleotide chicken microarray to assess the utility of cross species hybridisation (CSH). We successfully hybridised a number of different avian species to this array, obtaining reliable signals. We were able to distinguish ducks that were infected with avian influenza from uninfected ducks using this microarray platform. In addition, we were able to detect known chicken immunological genes in all of the hybridised avian species. CONCLUSION: Cross species hybridisation using long oligonucleotide microarrays is a powerful tool to study the immune response in avian species with little available genomic information. The present study validated the use of the whole genome long oligonucleotide chicken microarray to investigate gene expression in a range of avian species

VLT Diffraction Limited Imaging and Spectroscopy in the NIR: Weighing the black hole in Centaurus A with NACO

We present high spatial resolution near-infrared spectra and images of the nucleus of Centaurus A (NGC 5128) obtained with NAOS-CONICA at the VLT. The adaptive optics corrected data have a spatial resolution of 0.06" (FWHM) in K- and 0.11" in H-band, four times higher than previous studies. The observed gas motions suggest a kinematically hot disk which is orbiting a central object and is oriented nearly perpendicular to the nuclear jet. We model the central rotation and velocity dispersion curves of the [FeII] gas orbiting in the combined potential of the stellar mass and the (dominant) black hole. Our physically most plausible model, a dynamically hot and geometrically thin gas disk, yields a black hole mass of M_bh = (6.1 +0.6/-0.8) 10^7 M_sun. As the physical state of the gas is not well understood, we also consider two limiting cases: first a cold disk model, which completely neglects the velocity dispersion; it yields an M_bh estimate that is almost two times lower. The other extreme case is to model a spherical gas distribution in hydrostatic equilibrium through Jeans equation. Compared to the hot disk model the best-fit black hole mass increases by a factor of 1.5. This wide mass range spanned by the limiting cases shows how important the gas physics is even for high resolution data. Our overall best-fitting black hole mass is a factor of 2-4 lower than previous measurements. With our revised M_bh estimate, Cen A's offset from the M_bh-sigma relation is significantly reduced; it falls above this relation by a factor of ~2, which is close to the intrinsic scatter of this relation. (Abridged)Comment: 12 pages, 14 figures, including minor changes following the referee report; accepted for publication in The Astrophysical Journa

Principles for developing benchmark criteria for staff training in responsible gambling

One approach to minimizing the negative consequences of excessive gambling is staff training to reduce the rate of the development of new cases of harm or disorder within their customers. The primary goal of the present study was to assess suitable benchmark criteria for the training of gambling employees at casinos and lottery retailers. The study utilised the Delphi Method, a survey with one qualitative and two quantitative phases. A total of 21 invited international experts in the responsible gambling field participated in all three phases. A total of 75 performance indicators were outlined and assigned to six categories: (1) criteria of content, (2) modelling, (3) qualification of trainer, (4) framework conditions, (5) sustainability and (6) statistical indicators. Nine of the 75 indicators were rated as very important by 90 % or more of the experts. Unanimous support for importance was given to indicators such as (1) comprehensibility and (2) concrete action-guidance for handling with problem gamblers, Additionally, the study examined the implementation of benchmarking, when it should be conducted, and who should be responsible. Results indicated that benchmarking should be conducted every 1–2 years regularly and that one institution should be clearly defined and primarily responsible for benchmarking. The results of the present study provide the basis for developing a benchmarking for staff training in responsible gambling

The Dissociation between Early and Late Selection in Older Adults

Older adults exhibit a reduced ability to ignore task-irrelevant stimuli; however, it remains to be determined where along the information processing stream the most salient age-associated changes occur. In the current study, ERPs provided an opportunity to determine whether age-related differences in processing task-irrelevant stimuli were uniform across information processing stages or disproportionately affected either early or late selection. ERPs were measured in young and old adults during a color-selective attention task in which participants responded to target letters in a specified color (attend condition) while ignoring letters in a different color (ignore condition). Old participants were matched to two groups of young participants on the basis of neuropsychological test performance: one using age-appropriate norms and the other using test scores not adjusted for age. There were no age-associated differences in the magnitude of early selection (attend–ignore), as indexed by the size of the anterior selection positivity and posterior selection negativity. During late selection, as indexed by P3b amplitude, both groups of young participants generated neural responses to target letters under the attend versus ignore conditions that were highly differentiated. In striking contrast, old participants generated a P3b to target letters with no reliable differences between conditions. Individuals who were slow to initiate early selection appeared to be less successful at executing late selection. Despite relative preservation of the operations of early selection, processing delays may lead older participants to allocate excessive resources to task-irrelevant stimuli during late selection

Rapid phosphatidic acid accumulation in response to low temperature stress in Arabidopsis is generated through diacylglycerol kinase.

Phosphatidic acid (PtdOH) is emerging as an important signaling lipid in abiotic stress responses in plants. The effect of cold stress was monitored using (32)P-labeled seedlings and leaf discs of Arabidopsis thaliana. Low, non-freezing temperatures were found to trigger a very rapid (32)P-PtdOH increase, peaking within 2 and 5 min, respectively. In principle, PtdOH can be generated through three different pathways, i.e., (1) via de novo phospholipid biosynthesis (through acylation of lyso-PtdOH), (2) via phospholipase D hydrolysis of structural phospholipids, or (3) via phosphorylation of diacylglycerol (DAG) by DAG kinase (DGK). Using a differential (32)P-labeling protocol and a PLD-transphosphatidylation assay, evidence is provided that the rapid (32)P-PtdOH response was primarily generated through DGK. A simultaneous decrease in the levels of (32)P-PtdInsP, correlating in time, temperature dependency, and magnitude with the increase in (32)P-PtdOH, suggested that a PtdInsP-hydrolyzing PLC generated the DAG in this reaction. Testing T-DNA insertion lines available for the seven DGK genes, revealed no clear changes in (32)P-PtdOH responses, suggesting functional redundancy. Similarly, known cold-stress mutants were analyzed to investigate whether the PtdOH response acted downstream of the respective gene products. The hos1, los1, and fry1 mutants were found to exhibit normal PtdOH responses. Slight changes were found for ice1, snow1, and the overexpression line Super-ICE1, however, this was not cold-specific and likely due to pleiotropic effects. A tentative model illustrating direct cold effects on phospholipid metabolism is presented

Radiolysis of water ice in the outer solar system: Sputtering and trapping of radiation products

We performed quantitative laboratory radiolysis experiments on cubic water ice between 40 and 120 K, with 200 keV protons. We measured sputtering of atoms and molecules and the trapping of radiolytic molecular species. The experiments were done at fluences corresponding to exposure of the surface of the Jovian icy satellites to their radiation environment up to thousands of years. During irradiation, O2 molecules are ejected from the ice at a rate that grows roughly exponentially with temperature; this behavior is the main reason for the temperature dependence of the total sputtering yield. O2 trapped in the ice is thermally released from the ice upon warming; the desorbed flux starts at the irradiation temperature and increases strongly above 120 K. Several peaks in the desorption spectrum, which depend on irradiation temperature, point to a complex distribution of trapping sites in the ice matrix. The yield of O2 produced by the 200 keV protons and trapped in the ice is more than 2 orders of magnitude smaller than used in recent models of Ganymede. We also found small amounts of trapped H2O2 that desorb readily above 160 K.Fil: Bahr, D.A.. University of Virginia; Estados UnidosFil: Famá, M.. University of Virginia; Estados UnidosFil: Vidal, Ricardo Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; ArgentinaFil: Baragiola, Raul Antonio. University of Virginia; Estados Unido

Time-resolved broadband analysis of slow-light propagation and superluminal transmission of electromagnetic waves in three-dimensional photonic crystals

A time-resolved analysis of the amplitude and phase of THz pulses propagating through three-dimensional photonic crystals is presented. Single-cycle pulses of THz radiation allow measurements over a wide frequency range, spanning more than an octave below, at and above the bandgap of strongly dispersive photonic crystals. Transmission data provide evidence for slow group velocities at the photonic band edges and for superluminal transmission at frequencies in the gap. Our experimental results are in good agreement with finite-difference-time-domain simulations.Comment: 7 pages, 11 figure

Расчет ГИН по схеме Аркадьева-Маркса

Объектом исследования является генератор импульсного напряжения по схеме Аркадьева-Маркса. Цель работы: спроектировать и рассчитать генератор импульсных напряжений. В процессе работы рассчитаны количественные значения элементов ГИНа: значения коэффициентов использования разрядной схемы и волны; емкость и индуктивность конденсатора; количество ступеней; фронтовое и разрядное сопротивления. Был выполнен расчет ресурсов, ставки налогов, отчислений. Так же было описаны рабочее место и законодательные и нормативные документы.The object of study is the generator of pulse voltage according to the scheme Arkadiev-Marx. Objective: to design and calculate the voltage impulse generator. In the process, the calculated quantitative values of the elements of a Hin: the coefficients of use of the discharge circuit and wave; the capacitance and inductance of the capacitor; number of steps; the front and discharge resistors. Calculated resources, tax rates, deductions. As described workplace and legislative and regulatory documents